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Image Search Results
Journal: European journal of immunology
Article Title: Icaritin promotes tumor T-cell infiltration and induces antitumor immunity in mice.
doi: 10.1002/eji.201948225
Figure Lengend Snippet: Figure 4. Icaritin downregulates PD-L1 expression on MDSCs and neutrophils in the tumor microenvironment. C57BL/6 mice were treated with 70 mg/kg body weight of icaritin or vehicle control from day 3 to day 18 after the inoculation of B16F10 tumor cells. Tumors were digested into single cell suspensions and then subjected to surface staining. Percentage of Gr1+CD11b+ MDSCs in the tumor-infiltrating lymphocytes (A, B) and the surface expression level of PD-L1 on MDSCs (A, C, and D) from B16 tumors as compared to vehicle control were analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (E, F) Surface expression level of PD-L1 on neutrophils from PBMCs of icaritin-treated B16 mice as compared to vehicle control was analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (G, H) HL-60 cell lines were cultured with 1.25% DMSO for 6 days, TNF-α (G) and IL-6 (H) were used as positive controls for the induction of PD-L1 expression with or without the treatment of 20 µM icaritin for 24 h; PD-L1 expression level in the HL-60 cell lines was analyzed by western blot. Data are from one single experiment, representative of three independent experiments.
Article Snippet: Neutrophils from PBMCs were isolated by
Techniques: Expressing, Control, Staining, Cytometry, Cell Culture, Western Blot
Journal: Frontiers in Immunology
Article Title: Fibrocytes: A Critical Review and Practical Guide
doi: 10.3389/fimmu.2021.784401
Figure Lengend Snippet: Anti-collagen antibodies.
Article Snippet: ( ) , ICC ,
Techniques: Biomarker Discovery, Expressing, Cell Culture, In Situ Hybridization, Western Blot